quantum simply cellular anti-human igg kit  (Bangs Laboratories)

Journal: mAbs

Article Title: CC-96673 (BMS-986358), an affinity-tuned anti-CD47 and CD20 bispecific antibody with fully functional fc, selectively targets and depletes non-Hodgkin’s lymphoma

doi: 10.1080/19420862.2024.2310248

Figure Lengend Snippet: (c) Screening strategy for an optimally detuned anti-CD47 fab. A library of detuned CD47×EGFR bispecifics were screened for minimal binding to non-target CD47 + EGFR − Raji cells and maximum inhibition of SIRPα binding to CD47 + EGFR + FaDu cells. (d) Top: Representative curves of recombinant SIRPα binding to CD47 on the surface of each lymphoma cell line evaluated in the presence of CC-96673. Bottom: a summary of IC50 values and maximum percent inhibition of recombinant SIRPα binding to cell surface CD47 by CC-96673. The surface expression of CD47 and CD20 on NHL cell lines was determined using the QSC anti-human IgG kit and antibody-binding capacity (ABC) values were calculated automatically using the QuickCal template. Each symbol represents mean percent of control of SIRPα binding with error bars depicting standard deviation (SD) of two independent experiments.

Article Snippet: The surface expression of CD47 and CD20 on NHL cell lines was determined prior to the assessment of SIRPα binding competition using the Quantum Simply Cellular (QSC) anti-human IgG kit (Bangs Laboratories), as per the manufacturer’s instructions.

Techniques: Binding Assay, Inhibition, Recombinant, Expressing, Control, Standard Deviation